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e2f3 primary antibodies (Proteintech)

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Proteintech e2f3 primary antibodies

<t>E2F3</t> is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

E2f3 Primary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+e2f3+primary+antibody/E2F3+Antibody/pmc12801454-60-8-11
Average 93 stars, based on 22 article reviews

e2f3 primary antibodies - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis"

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

Journal: World Journal of Surgical Oncology

doi: 10.1186/s12957-025-04124-2

E2F3 is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

Figure Legend Snippet: E2F3 is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

Techniques Used: Binding Assay, Sequencing, Luciferase, Pull Down Assay, Western Blot, Over Expression

E2F3 overexpression reverses the effects of circ_0001741 knockdown A E2F3 overexpression partially rescues the anti-invasive effects of si-circ_0001741 or miR-194-5p in TE2 and KYSE150 cells B E2F3 overexpression partially restores proliferation suppressed by si-circ_0001741 or miR-194-5p. * P < 0.05

Figure Legend Snippet: E2F3 overexpression reverses the effects of circ_0001741 knockdown A E2F3 overexpression partially rescues the anti-invasive effects of si-circ_0001741 or miR-194-5p in TE2 and KYSE150 cells B E2F3 overexpression partially restores proliferation suppressed by si-circ_0001741 or miR-194-5p. * P < 0.05

Techniques Used: Over Expression, Knockdown

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Article Title: Identification of a novel prognostic signature composed of 3 cuproptosis-related transcription factors in colon adenocarcinoma.
Article Snippet: Background Since the mechanism of cuproptosis was recently revealed, many molecules related to this pathway have been widely concerned and exploited to have prognostic potential.. However, it is still unknown whether the transcription factors related to cuproptosis could be competent as tumor biomarkers of colon adenocarcinoma (COAD).. Objective To analyze the prognostic potential of cuproptosis-related transcription factors in COAD, and validate the representative molecule.

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Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 is a miR-194-5p target gene in TE2 and KYSE150 cells A Predicted partially complementary binding sequence between E2F3 mRNA and miR-194-5p B Dual-luciferase reporter assays confirming miR-194-5p binding to the E2F3 3’UTR. C Pull-down assay was used to confirm the interaction of E2F3 and miR-194-5p D Western blot analysis of E2F3 protein levels in TE2 cells after miR-194-5p overexpression. * P < 0.05

Article Snippet: Membranes were incubated overnight at 4 °C with E2F3 primary antibodies (Proteintech, China, Cat No. 27615-1-AP), followed by HRP-conjugated secondary antibodies (Proteintech, China).

Techniques: Binding Assay, Sequencing, Luciferase, Pull Down Assay, Western Blot, Over Expression

Journal: World Journal of Surgical Oncology

Article Title: Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis

doi: 10.1186/s12957-025-04124-2

Figure Lengend Snippet: E2F3 overexpression reverses the effects of circ_0001741 knockdown A E2F3 overexpression partially rescues the anti-invasive effects of si-circ_0001741 or miR-194-5p in TE2 and KYSE150 cells B E2F3 overexpression partially restores proliferation suppressed by si-circ_0001741 or miR-194-5p. * P < 0.05

Techniques: Over Expression, Knockdown

The impact of Brevilin A on lncRNA H19, miR-194, and E2F3 expressions. DU145 cells were treated at the concentrations of 10 μM and 20 μM, which were calculated through the IC 50 values of Brevilin A. ( A – I ) qRT-PCR determined lncRNA H19 ( A – C ), miR-194 ( D – F ) and E2F3 mRNA ( G – I ) expressions. ( J – L ) Western blot measured E2F3 expression. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. control), n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The impact of Brevilin A on lncRNA H19, miR-194, and E2F3 expressions. DU145 cells were treated at the concentrations of 10 μM and 20 μM, which were calculated through the IC 50 values of Brevilin A. ( A – I ) qRT-PCR determined lncRNA H19 ( A – C ), miR-194 ( D – F ) and E2F3 mRNA ( G – I ) expressions. ( J – L ) Western blot measured E2F3 expression. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. control), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

The profiles of lncRNA H19, miR-194, and E2F3 in prostate cancer tissues and cell lines. ( A , B ) qRT-PCR detected lncRNA H19 expression in prostate cancer tissues and cell lines. ( C , D ) qRT-PCR determined miR-194 expression in prostate cancer tissues and cell lines. ( E , F ) qRT-PCR examined E2F3 mRNA expression in prostate cancer tissues and cell lines. ( G , H ) Western blot was performed for assaying E2F3 protein level. ( I ) Immunohistochemistry checked E2F3 expression in prostate cancer tissues. Scale bar = 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. Normal or PrEC), n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The profiles of lncRNA H19, miR-194, and E2F3 in prostate cancer tissues and cell lines. ( A , B ) qRT-PCR detected lncRNA H19 expression in prostate cancer tissues and cell lines. ( C , D ) qRT-PCR determined miR-194 expression in prostate cancer tissues and cell lines. ( E , F ) qRT-PCR examined E2F3 mRNA expression in prostate cancer tissues and cell lines. ( G , H ) Western blot was performed for assaying E2F3 protein level. ( I ) Immunohistochemistry checked E2F3 expression in prostate cancer tissues. Scale bar = 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. Normal or PrEC), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemistry

The interaction among lncRNA H19, miR-194, and E2F3. ( A , B ) Starbase database the binding site between lncRNA H19 and miR-194 as well as the binding site between miR-194 and E2F3. ( C – F ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( G , H ) Dual luciferase reporter gene assay measured luciferase activity of DU145 cells. ( I , J ) The miR-194 level was probed via qRT-PCR. ( K – R ) Western blot and qRT-PCR tested the effects of lncRNA H19 and miR-194 on E2F3 expression. ns P > 0.05, *** P < 0.001, n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The interaction among lncRNA H19, miR-194, and E2F3. ( A , B ) Starbase database the binding site between lncRNA H19 and miR-194 as well as the binding site between miR-194 and E2F3. ( C – F ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( G , H ) Dual luciferase reporter gene assay measured luciferase activity of DU145 cells. ( I , J ) The miR-194 level was probed via qRT-PCR. ( K – R ) Western blot and qRT-PCR tested the effects of lncRNA H19 and miR-194 on E2F3 expression. ns P > 0.05, *** P < 0.001, n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Gene Assay, Activity Assay, Western Blot, Expressing

The influence of lncRNA H19 and miR-194 on DU145 cell proliferation, apoptosis, invasion, and migration. H19 overexpression plasmids and si-H19 were transfected into DU145 cells, and miR-194 mimics or inhibitors were administered for 24 hours culture. ( A , B ) CCK8 assay detected cell proliferation. ( C , D ) Transwell monitored cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining examined apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC or siNC); && P < 0.01, &&& P < 0.001 (vs. H19 or siH19), n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The influence of lncRNA H19 and miR-194 on DU145 cell proliferation, apoptosis, invasion, and migration. H19 overexpression plasmids and si-H19 were transfected into DU145 cells, and miR-194 mimics or inhibitors were administered for 24 hours culture. ( A , B ) CCK8 assay detected cell proliferation. ( C , D ) Transwell monitored cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining examined apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC or siNC); && P < 0.01, &&& P < 0.001 (vs. H19 or siH19), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Migration, Over Expression, Transfection, CCK-8 Assay, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

The impact of miR-194 and E2F3 on DU145 cell proliferation, apoptosis, invasion, and migration. E2F3 overexpression and si-E2F3 plasmids were transfected into DU145 cells for 24 hour culture after miR-194 mimics or inhibitors were administered. ( A , B ) CCK8 assay checked cell proliferation. ( C , D ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC or NC-in); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. miR-149 or miR-149 in), n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The impact of miR-194 and E2F3 on DU145 cell proliferation, apoptosis, invasion, and migration. E2F3 overexpression and si-E2F3 plasmids were transfected into DU145 cells for 24 hour culture after miR-194 mimics or inhibitors were administered. ( A , B ) CCK8 assay checked cell proliferation. ( C , D ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( E , F ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( G , H ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( I , J ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC or NC-in); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. miR-149 or miR-149 in), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Migration, Over Expression, Transfection, CCK-8 Assay, Transwell Assay, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

Brevilin A hampered the pro-cancer function of lncRNA H19. Brevilin A was utilized to treat DU145 cells stably transfected with lncRNA H19 overexpression plasmids. ( A ) CCK8 assay checked cell proliferation. ( B ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( C ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( D , E ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( F ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. H19), n = 3.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: Brevilin A hampered the pro-cancer function of lncRNA H19. Brevilin A was utilized to treat DU145 cells stably transfected with lncRNA H19 overexpression plasmids. ( A ) CCK8 assay checked cell proliferation. ( B ) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. ( C ) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. ( D , E ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( F ) Western blot was used for examining E2F3 protein level. ** P < 0.01, *** P < 0.001 (vs. NC); & P < 0.05, && P < 0.01, &&& P < 0.001 (vs. H19), n = 3.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Stable Transfection, Transfection, Over Expression, CCK-8 Assay, Transwell Assay, Migration, TUNEL Assay, Staining, Quantitative RT-PCR, Western Blot

The functions of Brevilin A and lncRNA H19 in the prostate cancer nude mouse xenograft model. DU145 cells, stably transfected with lncRNA H19 overexpression plasmids, were taken to construct a nude mouse xenograft model of prostate cancer, and Brevilin A was harnessed for treatment. The tumors were excised 28 days later. ( A ) The tumor volume. ( B ) The images of tumors. ( C ) The tumor masses. ( D , E ) Immunohistochemistry determined Ki67 and E2F3 expressions. ( F , G ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( H ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC); && P < 0.01, &&& P < 0.001 (vs. H19), n = 5.

Journal: Aging (Albany NY)

Article Title: Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

doi: 10.18632/aging.204744

Figure Lengend Snippet: The functions of Brevilin A and lncRNA H19 in the prostate cancer nude mouse xenograft model. DU145 cells, stably transfected with lncRNA H19 overexpression plasmids, were taken to construct a nude mouse xenograft model of prostate cancer, and Brevilin A was harnessed for treatment. The tumors were excised 28 days later. ( A ) The tumor volume. ( B ) The images of tumors. ( C ) The tumor masses. ( D , E ) Immunohistochemistry determined Ki67 and E2F3 expressions. ( F , G ) qRT-PCR examined the profiles of lncRNA H19 and miR-194. ( H ) Western blot was used for examining E2F3 protein level. *** P < 0.001 (vs. NC); && P < 0.01, &&& P < 0.001 (vs. H19), n = 5.

Article Snippet: Primary anti-E2F3 antibody (1:100) (Proteintech, Wuhan, China) or Ki67 antibody (1:100) (Abcam, ab15580, MA, USA) was added for maintaining at 4°C overnight.

Techniques: Stable Transfection, Transfection, Over Expression, Construct, Immunohistochemistry, Quantitative RT-PCR, Western Blot

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e2f3 primary antibodies (Proteintech)

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1) Product Images from "Circ_0001741 regulates proliferation and invasion in ESCC via the miR-194-5p/E2F3 axis"

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